Device Foundry (Bahen)

Instrument Name Room Academic Hourly Rate Part of Monthly CAP? Short Description Funding
EVG 520 (6 inch) BA 7175a $50/hr Yes Semi-automated Hot Embossing System for embossing/bonding
Supports up to 6 in wafers
Maximum Temperature: 350 °CMaximum Contact Force: 20KN		 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
EVG 520 (8 inch) BA 7175b $60/hr Yes Semi-automated Hot Embossing System for embossing/bonding
Supports up to 8 in wafers
Up to 6 inch substrates
Maximum Contact Force: 100KN		 Living Micro-Systems Foundry, Health Challenge Program, National Research Council of Canada, #CH-2019-1-0002
OAI Mask Aligner BA 7175a $30/hr Yes Mask Aligner primarily for flood exposure
Up to 4 inch substrates
lamps ranging: 200 to 2000W
Timer: 0.1 to 99.0 sec. at 0.1 second increments
EVG 620 Mask Aligner BA 7175a $45/hr Yes Mask Aligner for multi-layer alignment
4′ substrate holder with 5″ photomask holder
6″ substrate chuck with 7″ photomask holder
Exposure source: UV LED light source		 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
upg501 Mask Writer BA 7175a $25/hr Yes For chrome-on-glass photomasks with resolution down to 1 um. The system uses 392nm source.

SOP- uPG501 Mask Writer

 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
Hitachi TM4000 SEM BA 7175a $50/hr Yes Scanning Electron Microscope
Sample sizes to 80 mm (diameter) and 50 mm (height)
Accelerating Voltage: 5,10,15 kV		 Living Micro-Systems Foundry, Health Challenge Program, National Research Council of Canada, #CH-2019-1-0002
Leica Sputter Coater BA 7175a $75/hr Yes High vacuum film deposition system
Fully automated coating process
Targets available: gold, gold palladium, aluminum, platinum, chrome, and carbon thread		 The CenteR for Advancing Neurotechnological Innovation to Application, Canada Foundation for Innovation/Ontario Research Fund, #302788
3D Printer (3500HD Max) BA 7175d Contact Staff No MultiJet Printing plastic 3D printer
50um XY resolution, 16um Z resolution
minimum feature size ~150umAcrylic resin part with parrafin wax support material
Accepts .STL files
CO2 Laser Cutter BA 7175b $50/hr Yes 50W CO2 laser
Spot size ~200um
For etching/cutting polymers (ideal for acrylics)
Accepts CAD files		 The CenteR for Advancing Neurotechnological Innovation to Application, Canada Foundation for Innovation/Ontario Research Fund, #302788
ML3 Baby Direct Writer (385nm) BA 7175a $30/hr Yes Direct-write photolithography machine
385nm long-life semiconductor light source155mm x 155mm x 7mm maximum wafer size
1µm minimum feature sizeSpeed: up to 50mm^2/minute
Accepts CIF, GDS2, BMP, TIFF, JPEG, PNG, GIF		 “Microfabrication Equipment for Magnetic Micro-Mechanical Structures” Research Tools and Instruments, Natural Sciences and Engineering Research Council of Canada, RTI-2017-00456
ML3 Pro Direct Writer (365nm) BA 7175a $30/hr Yes Direct-write photolithography machine for rapid prototyping
365nm long-life semiconductor light source195mm x 195mm maximum writing area
230mm x 230mm x 15mm maximum wafer size0.6µm minimum feature sizes
Speed: up to 17mm^2/minute		 The CenteR for Advancing Neurotechnological Innovation to Application, Canada Foundation for Innovation/Ontario Research Fund, #302788
SU8 Spinner (6 inch) BA 7175a $10/hr Yes Used for SU-8 spinning on silicon wafer
Speed: 1-4000 RPM
Acceleration or 1 rpm/second increments. Up to 6 inch substratesSOP – Spin Coater		 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
SU8 Spinner (8-inch) BA 7175a $15/hr Yes Used for SU-8 spinning on silicon wafer
Speed: 1-5000 RPM
Acceleration or 1 rpm/second increments
Up to 8 inch substratesSOP – Spin Coater
Non-SU8 Spinner (6-inch) BA 7175a $10/hr Yes Used for SU-8 spinning on silicon wafer
Speed: 1-4000 RPM
Acceleration or 1 rpm/second increments
Up to 6 inch substratesSOP – Spin Coater		 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
Non-SU8 Spinner (8-inch) BA 7175a $15/hr Yes Used for SU-8 spinning on silicon wafer
Speed: 1-5000 RPM
Acceleration or 1 rpm/second increments
Up to 8 inch substratesSOP – Spin Coater
Hot Plate 1-4 (6-inch) BA 7175a $15/hr Yes Programmable with 9’x9′ area Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
Hot Plate 1-3 (8-inch) BA 7175a $20/hr Yes Programmable with 12’x12′ area
Optical Profiler BA 7175a $30/hr Yes Optical profiler for 3D surface measurements. Ideal for flat reflective surfaces. Sub-micron step height resolution. Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
Carver Press BA 7175b $25/hr Heating up to 250C
Clamping force: 5000lb
6×6″ platen size
No active cooling
Roll-2-Roll Polymer Coater BA 7175b Contact Staff No Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
Corona Treater BA 7175d $20/hr Yes For activation of PDMS/glass surfaces
Plasma Oven BA 7175a $10/hr Yes Plasma cleaner for activation of PDMS/glass surfaces
6″ diameter x 6.5″ length Pyrex chamber
Maximum RF power of 45W
Untilizes air plasma (no O2 available)		 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
CAD Station BA 7175 $25/hr Yes PC Workstation that gives users access to AutoCAD, SolidWorks, CleWin, DScribe, and KLayout. Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
Injection Molder BA 7175b Contact Staff No Wittmann Battenfeld MicroPower 15.

15 Ton injection molder equipped with 4-inch mold and 1×2″ MF device insert.

Produces parts out of various thermoplastics.

 Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
LightFAB 3D Glass Printer BA 7175 $80/hr Yes 3D Printer for fabricating glass MF devices, stamps, molds, etc.

Resolution down to 1um.

The printer can also weld glass.

 Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
Nanoscribe GT2 3D Printer BA 7175a $20/hr Yes 3D Printer capable of printing down to 200nm in various materials using layer-by-layer 2-photon polymerization. Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
Keyence Digital Microscope BA 7175 $40/hr Yes Keyence VHX7000 microscope system for large FOV, high depth of focus, oblique imaging and profiling. Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
Parylene Coater BA 7175 $40/hr Yes SCS LabCoater 2 (PDS 2010)
Datron Neo CNC Mill BA 7175 $50/hr Yes Datron Neo Series 2 Micro Milling Machine Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442

Tissue Foundry (MC)

Instrument Name Room Academic Rate Description Funding
Thermal Cycler MC 403 $8/hr Model: Bio-Rad T100 Thermal Cycler

Applications: Nucleic acid amplification (PCR), Gene cloning and analysis, Gene expression analysis, Mutational analysis, Cycle sequencing
Sample capacity: 96 x 0.2 ml tubes, 0.2 ml tube strips, or 1 x 96-well plate
Temperature range: 4 –100°C
Gradient range: 30–100°C

SOP – Thermal Cycler

 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
PCR (96 Well Plate) MC 403 $8/hr Model: Bio-Rad CFX96

Real-time PCR
Accomodated 96 well plate
Maximum 5-target per well
Sample volumes: 1–50 (10–25 recommended)

SOP – Thermal Cycler

 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
PCR (384 Well Plate) MC 403 $8/hr Model: Bio-Rad CFX384

Real-time PCR
Accomodated 384 well plate
Maximum 4-target per well
Minimum sample volumes: 1–30 (5–20 recommended)

SOP – Thermal Cycler

 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
Confocal Microscope MC 403 $20/hr Model: Nikon A1 Confocal

  • Brightfield
  • Fluorescent (4 filter cubes)
  • Confocal (4 lasers)
  • 4x, 10x, 20x, 40x, 60x objectives available
  • DIC, Polarization, and Reflectance Modes

SOP – Nikon Confocal

 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
Atomic Force Microscope MC 403 $30/hr Model: Bruker BioScope Catalyst

Integration of AFM imaging with optical imaging – Designed for biological samples
X-Y Scan Range: 150µm, open-loop or closed-loop operation
Z Scan Range: 20µm open-loop or closed-loop operation
Deflection Detection IR super luminescent diode (SLD ): 850nm

 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
Zeiss Live Cell Imaging Microscope MC 403 $20/hr Model: Zeiss AxioObserver

Inverted wide field flourescent microscope for live cell imaging with integrated environmental chamber.
Flourescent Channels: DAPI, TRITC,FITC, Cy5
Objectives: Brightfield, 4, 10, 20x

SOP – Zeiss Microscope

 Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
Interfacial Hybrid Rheometer MC 403 $50/hr TA Instruments DHR3 Hybrid Rheometer

Minimum Torque (nN.m):0.5 (Oscillation), 5 (Steady Shear) – 200
Angular Velocity (rad/s):0 – 300

Accessories:

Interfacial chamber, Peltier plate (heated/cooled), microscope accessory,

Available geometries:

  1. 60mm flat plate
  2. 60 mm sand-blasted flat plate
  3. 40mm flat plate
  4. 60mm 1 degree cone

SOP – Interfacial Hybrid Rheometer

Additional Guides

 Enabling the discovery and development of functional materials and technologies via confocal rheometry. Research Tools and Instruments, Natural Sciences and Engineering Research Council of Canada, RTI-2018-00878
IncuCyte S3 Imaging System MC 403 $10/hr Real-time live-cell analysis system for monitoring and surveillance
Exported Image Format: JPEG, PNG, TIFF
Fluorescence Channels: Green, Red
Objectives 4,10,20X		 Living Micro-Systems Foundry, Health Challenge Program, National Research Council of Canada, #CH-2019-1-0002
RegenHu Discovery 3D Bioprinter MC 403 $25/hr Model: RegenHu 3D Discovery

3-axis 3D Bioprinter
UV curing kit:365nm with a maximal output of 500mW
Printhead:
Cell extrusion

 “3D Printing and Biofabrication Instrument” Research Tools and Instruments, Natural Sciences and Engineering Research Council of Canada, RTI-2015-473509
BioAssemblyBot 3D Bioprinter MC 403 $25/hr Advanced Solutions BioAssemblyBot

6-axis 3D Bioprinter
3D Scanner
Heated and cooled stage
Printheads available:
Heating, Cooling, UV
Single and dual pnematic extruder
Co-axial extruder

 Living Micro-Systems Foundry, Health Challenge Program, National Research Council of Canada, #CH-2019-1-0002
Biosafety Cabinets MC 403 Five BSCs available (4 foot and 6 foot)-

SOP – BSCs
CO2 Incubators MC 403 – Nine incubators available

SOP – Incubators
Fume Hoods MC 405 – Two available
Cell Counter MC 405 $20/hr Bio-Rad TC20 Automated Cell Counter Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707
IMARIS Workstation MC 403 $40/hr IMARIS Image quantification and analysis software – comes with all features including: MeasurementPro, ClearView Deconvolution, ImarisTrackLineage, ImarisColoc, ImarisXT, ImarisCell, FilamentTracer, ImarisVantage, ImarisBatch and ImarisStitcher Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
BioTester 5000 MC 405 $20/hr Bi-Axial mechanical tester for samples, with various BioRake mounting accessories.

  • Long stroke for up to 80mm grip separation.
  • Temperature control

SOP – BioTester 5000
Leica Stellaris 5 LightSheet/Confocal Microscope MC 403 $40/hr Leica Stellaris 5

  • Confocal + light sheet microscopy in one system
  • Brightfield/fluorescent light source available
  • 6 lasers (405 nm, 448 nm, 488 nm, 514 nm, 561 nm, 638 nm)
  • 5 detectors (can image 5 channels simultaneously)
  • Confocal objectives: 1.6x, 5x, 10x, 20x, 40x w, 63x oil. (available but not installed 2.5x and 25x)
  • Light sheet detection objectives: 5x, 10x, 16x
  • OkoLabs full environmental chamber (temp. up to 55C, CO2, humidity).

SOP – Leica Stellaris 5 Confocal

 Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
High Content Confocal Imaging System (Perkin Elmer Operetta) MC 405 $20/hr (80% discount on +24 hours experiments) Four component system consisting of:

1. Perkin Elmer Operetta:

  • Spinning disk confocal
  • Brightfield and fluorescence
  • 8 lasers (365, 440, 475, 510, 550, 580, 630, 660)
  • Objectives: 5x, 10x, 20x, 40x (water)
  • Environmental chamber: CO2, humidity, temp control

2. JANUS G3 Automated Liquid Handler

3. Automated Incubator

4. Robotic Arm for sample manipulation

 Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442
Autoclave MC405/403 Free SOP – Autoclave