Instruments
Documentation and specifications for CRAFT’s research instruments. Photos and details are listed below.
| Instrument Name | Room | Academic Hourly Rate | Part of Monthly CAP? | Short Description | Funding | |
|---|---|---|---|---|---|---|
 | EVG 520 (6 inch) | BA 7175a | $50/hr | Yes | Semi-automated Hot Embossing System for embossing/bonding Supports up to 6 in wafers Maximum Temperature: 350 °C Maximum Contact Force: 20KN | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | EVG 520 (8 inch) | BA 7175b | $60/hr | Yes | Semi-automated Hot Embossing System for embossing/bonding Supports up to 8 in wafers Up to 6 inch substrates Maximum Contact Force: 100KN | Living Micro-Systems Foundry, Health Challenge Program, National Research Council of Canada, #CH-2019-1-0002 |
 | OAI Mask Aligner | BA 7175a | $30/hr | Yes | Mask Aligner primarily for flood exposure Up to 4 inch substrates lamps ranging: 200 to 2000W Timer: 0.1 to 99.0 sec. at 0.1 second increments | |
 | EVG 620 Mask Aligner | BA 7175a | $45/hr | Yes | Mask Aligner for multi-layer alignment 4′ substrate holder with 5″ photomask holder 6″ substrate chuck with 7″ photomask holder Exposure source: UV LED light source | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | upg501 Mask Writer | BA 7175a | $25/hr | Yes | For chrome-on-glass photomasks with resolution down to 1 um. The system uses 392nm source. SOP- uPG501 Mask Writer | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | Hitachi TM4000 SEM | BA 7175a | $50/hr | Yes | Scanning Electron Microscope Sample sizes to 80 mm (diameter) and 50 mm (height) Accelerating Voltage: 5,10,15 kV | Living Micro-Systems Foundry, Health Challenge Program, National Research Council of Canada, #CH-2019-1-0002 |
 | Leica Sputter Coater | BA 7175a | $75/hr | Yes | High vacuum film deposition system Fully automated coating process Targets available: gold, gold palladium, aluminum, platinum, chrome, and carbon thread | The Center for Advancing Neurotechnological Innovation to Application, Canada Foundation for Innovation/Ontario Research Fund, #302788 |
 | 3D Printer (3500HD Max) | BA 7175d | Contact Staff | No | MultiJet Printing plastic 3D printer 50um XY resolution, 16um Z resolution minimum feature size ~150um Acrylic resin part with parrafin wax support material Accepts .STL files | |
 | CO2 Laser Cutter | BA 7175b | $50/hr | Yes | 50W CO2 laser Spot size ~200um For etching/cutting polymers (ideal for acrylics) Accepts CAD files | The Center for Advancing Neurotechnological Innovation to Application, Canada Foundation for Innovation/Ontario Research Fund, #302788 |
 | ML3 Baby Direct Writer (385nm) | BA 7175a | $30/hr | Yes | Direct-write photolithography machine 385nm long-life semiconductor light source 155mm x 155mm x 7mm maximum wafer size 1µm minimum feature size Speed: up to 50mm^2/minute Accepts CIF, GDS2, BMP, TIFF, JPEG, PNG, GIF | “Microfabrication Equipment for Magnetic Micro-Mechanical Structures” Research Tools and Instruments, Natural Sciences and Engineering Research Council of Canada, RTI-2017-00456 |
| ML3 Pro Direct Writer (365nm) | BA 7175a | $30/hr | Yes | Direct-write photolithography machine for rapid prototyping 365nm long-life semiconductor light source 195mm x 195mm maximum writing area 230mm x 230mm x 15mm maximum wafer size 0.6µm minimum feature sizes Speed: up to 17mm^2/minute | The Center for Advancing Neurotechnological Innovation to Application, Canada Foundation for Innovation/Ontario Research Fund, #302788 |
 | SU8 Spinner (6 inch) | BA 7175a | $10/hr | Yes | Used for SU-8 spinning on silicon wafer Speed: 1-4000 RPM Acceleration or 1 rpm/second increments. Up to 6 inch substrates SOP – Spin Coater | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | SU8 Spinner (8-inch) | BA 7175a | $15/hr | Yes | Used for SU-8 spinning on silicon wafer Speed: 1-5000 RPM Acceleration or 1 rpm/second increments Up to 8 inch substrates SOP – Spin Coater | |
| Non-SU8 Spinner (6-inch) | BA 7175a | $10/hr | Yes | Used for SU-8 spinning on silicon wafer Speed: 1-4000 RPM Acceleration or 1 rpm/second increments Up to 6 inch substrates SOP – Spin Coater | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
| Non-SU8 Spinner (8-inch) | BA 7175a | $15/hr | Yes | Used for SU-8 spinning on silicon wafer Speed: 1-5000 RPM Acceleration or 1 rpm/second increments Up to 8 inch substrates SOP – Spin Coater | |
 | Hot Plate 1-4 (6-inch) | BA 7175a | $15/hr | Yes | Programmable with 9’x9′ area | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | Hot Plate 1-3 (8-inch) | BA 7175a | $20/hr | Yes | Programmable with 12’x12′ area | |
 | Optical Profiler | BA 7175a | $30/hr | Yes | Optical profiler for 3D surface measurements. Ideal for flat reflective surfaces. Sub-micron step height resolution. | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
| Carver Press | BA 7175b | $25/hr | Heating up to 250C Clamping force: 5000lb 6×6″ platen size No active cooling | |||
 | Roll-2-Roll Polymer Coater | BA 7175b | Contact Staff | No | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 | |
| Corona Treater | BA 7175d | $20/hr | Yes | For activation of PDMS/glass surfaces | ||
 | Plasma Oven | BA 7175a | $10/hr | Yes | Plasma cleaner for activation of PDMS/glass surfaces 6″ diameter x 6.5″ length Pyrex chamber Maximum RF power of 45W Utilizes air plasma (no O2 available) | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | CAD Station | BA 7175 | $25/hr | Yes | PC Workstation that gives users access to AutoCAD, SolidWorks, CleWin, DScribe, and KLayout. | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
 | Injection Molder | BA 7175b | Contact Staff | No | Wittmann Battenfeld MicroPower 15. 15 Ton injection molder equipped with 4-inch mold and 1×2″ MF device insert. Produces parts out of various thermoplastics. | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
 | LightFAB 3D Glass Printer | BA 7175 | $80/hr | Yes | 3D Printer for fabricating glass MF devices, stamps, molds, etc. Resolution down to 1um. The printer can also weld glass. | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
 | Nanoscribe GT2 3D Printer | BA 7175a | $20/hr | Yes | 3D Printer capable of printing down to 200nm in various materials using layer-by-layer 2-photon polymerization. | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
 | Keyence Digital Microscope | BA 7175 | $40/hr | Yes | Keyence VHX7000 microscope system for large FOV, high depth of focus, oblique imaging and profiling. | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
 | Parylene Coater | BA 7175 | $40/hr | Yes | SCS LabCoater 2 (PDS 2010) | |
 | Datron Neo CNC Mill | BA 7175 | $75/hr | Yes | Datron Neo Series 2 Micro Milling Machine | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
| Instrument Name | Room | Academic Rate | Description | Funding | |
|---|---|---|---|---|---|
 | Thermal Cycler | MC 403 | $8/hr | Model: Bio-Rad T100 Thermal Cycler Applications: Nucleic acid amplification (PCR), Gene cloning and analysis, Gene expression analysis, mutational analysis, cycle sequencing Sample capacity: 96 x 0.2 mL tubes, 0.2 mL tube strips, or 1 x 96-well plate Temperature range: 4 –100°C Gradient range: 30–100°C SOP – Thermal Cycler: SOP – Thermal Cycler | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | PCR (96 Well Plate) | MC 403 | $8/hr | Model: Bio-Rad CFX96 Real-time PCR accommodates 96-well plates Maximum 5-target per well Sample volumes: 1–50 µL (10–25 µL recommended) SOP – Thermal Cycler: SOP – Thermal Cycler | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | PCR (384 Well Plate) | MC 403 | $8/hr | Model: Bio-Rad CFX384 Real-time PCR accommodates 384-well plates Maximum 4-target per well Sample volumes: 1–30 µL (5–20 µL recommended) SOP – Thermal Cycler: SOP – Thermal Cycler | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | Confocal Microscope | MC 403 | $20/hr | Model: Nikon A1 Confocal
SOP – Nikon Confocal: SOP – Nikon Confocal | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | Atomic Force Microscope | MC 403 | $30/hr | Model: Bruker BioScope Catalyst Integration of AFM imaging with optical imaging – designed for biological samples XY scan range: 150 µm, open-loop or closed-loop operation Z scan range: 20 µm, open-loop or closed-loop operation Deflection detection IR superluminescent diode (SLD): 850 nm Available geometries:
SOP – Interfacial Hybrid Rheometer: SOP – Interfacial Hybrid Rheometer | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707; Engineering Research Council of Canada, RTI-2018-00879 |
 | IncuCyte S3 Imaging System | MC 403 | $10/hr | Real-time live-cell analysis system for monitoring and surveillance Exported image formats: JPEG, PNG, TIFF Fluorescence channels: Green, Red Objectives: 4x, 10x, 20x | Living Micro-Systems Foundry, Health Challenge Program, National Research Council of Canada, #CH-2019-1-0002 |
 | RegenHu Discovery 3D Bioprinter | MC 403 | $25/hr | Model: RegenHu 3D Discovery 3-axis 3D bioprinter UV curing kit: 365 nm with a maximal output of 500 mW Printhead: Cell extrusion | “3D Printing and Biofabrication Instrument” Research Tools and Instruments, Natural Sciences and Engineering Research Council of Canada, RTI-2015-473509 |
 | BioAssemblyBot 3D Bioprinter | MC 403 | $25/hr | Model: Advanced Solutions BioAssemblyBot 6-axis 3D bioprinter with 3D scanner Heated and cooled stage Printheads available: heating, cooling, UV, single and dual pneumatic extruders, co-axial extruder | Living Micro-Systems Foundry, Health Challenge Program, National Research Council of Canada, #CH-2019-1-0002 |
 | Biosafety Cabinets | MC 403 | N/A | Five BSCs available (4-foot and 6-foot) SOP – BSCs: SOP – BSCs | N/A |
 | CO2 Incubators | MC 403 | N/A | Nine incubators available SOP – Incubators: SOP – Incubators | N/A |
 | Fume Hoods | MC 405 | N/A | Two available | N/A |
 | Cell Counter | MC 405 | $20/hr | Model: Bio-Rad TC20 Automated Cell Counter | Centre for Microfluidic Systems in Chemistry and Biology, Canada Foundation for Innovation/Ontario Research Fund, #21707 |
 | IMARIS Workstation | MC 403 | $40/hr | IMARIS image quantification and analysis software – comes with all features including: MeasurementPro, ClearView Deconvolution, ImarisTrackLineage, ImarisColoc, ImarisXT, ImarisCell, FilamentTracer, ImarisVantage, ImarisBatch, and ImarisStitcher | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
 | BioTester 5000 | MC 405 | $20/hr | Bi-axial mechanical tester for samples, with various BioRake mounting accessories.
SOP – BioTester 5000: SOP – BioTester 5000 | N/A |
 | Leica Stellaris 5 Lightsheet/Confocal Microscope | MC 403 | $40/hr | Leica Stellaris 5 – Confocal + light sheet microscopy in one system
SOP – Leica Stellaris 5 Confocal: SOP – Leica Stellaris 5 Confocal | Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
 | High Content Confocal Imaging System (Perkin Elmer Operetta) | MC 405 | $20/hr (80% discount on ≥24 hours experiments) | Four-component system consisting of:
| Ontario-Quebec Center for Organ-on-a-Chip Engineering, Canada Foundation for Innovation/Ontario Research Fund, #36442 |
| Autoclave | MC 405/403 | Free | SOP – Autoclave: SOP – Autoclave | N/A |